Extended Data Fig. 3: SIV-associated genes and IL-15 subunit genes show a transient change in expression after treatment with N-803 alone.
From: Robust and persistent reactivation of SIV and HIV by N-803 and depletion of CD8+ cells
RNA was extracted from sorted peripheral bulk CD4+ T cells (CD3+CD4+CD8−CD20−CD14−) (left), bulk CD8+ T cells (CD3+CD4−CD8+CD20−CD14−) (middle) and NK cells (CD3−CD20−CD14−NKG2A+) (right) and libraries were prepared, normalized, pooled and clustered on flow cells for sequencing. RNA-sequencing data were aligned to the MacaM v.7.8 assembly of the Indian rhesus macaque genome. Transcripts were analysed for alignment against a custom gene set with SIV host restriction factors, PPIA (capsid folding protein), SIV receptors, SIV receptor agonists, NF-κB subunits (involved in mediating the transcription of long-terminal repeat regions), IL-15 receptor subunits and NFAT subunits. D, day; W, week.
