Extended Data Fig. 7: AZD5582-induced gene pathways and genes in SIV-infected ART-suppressed rhesus macaques.
From: Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo
a, DAVID analysis showing pathways significantly enriched in genes differentially expressed after AZD5582 treatment relative to baseline in CD4+ T cells isolated from lymph nodes (black bars) and peripheral blood (grey bars). n = 6 for both lymph nodes and peripheral blood; for each, n = 3 for 3 doses of AZD5582 and n = 3 for 10 doses of AZD5582. b, Leading edge genes from the ‘hallmark TNF signalling via NF-κB’ pathway from MSigDB. Genes were identified in the leading edge of CD4+ T cell samples from the lymph nodes before and after treatment with AZD5582 (shown in Fig. 4b). The contrast depicted is the fold change of each gene for each rhesus macaque’s post-treatment sample relative to the pre-treatment values for CD4+ T cells from the lymph nodes (top) and the peripheral blood (bottom). n = 6 for both lymph nodes and peripheral blood; for each, n = 3 for 3 doses of AZD5582 and n = 3 for 10 doses of AZD5582. c, Heat map of cNF-κB (top) and ncNF-κB (bottom) pathway gene expression in rhesus macaques with (left, n = 5) or without (right, n = 6) on-ART viraemia of >60 copies per ml plasma. One rhesus macaque without on-ART viraemia was excluded from this analysis because of technical issues (higher than expected unmapped and multi-mapped reads, and lower than expected unique identified reads compared to the means). Colour scale, log2-transformed fold changes of post-treatment compared with pre-treatment values.
