Extended Data Fig. 3: AZD5582 ex vivo target engagement.
From: Systemic HIV and SIV latency reversal via non-canonical NF-κB signalling in vivo
a, Western blot analysis of p100, p52 and cIAP1 protein levels in cells isolated from the bone marrow, lymph node and spleen of BLT mice before and 20 h after ex vivo treatment with AZD5582. Loading control, β-actin. Representative of two experiments. b, cIAP expression in resting CD4+ T cells isolated from the thymus, spleen, lymph nodes, liver, lung and bone marrow. Loading control, β-actin. Representative of two experiments. c, cIAP expression in the thymic organoid of HIV-infected ART-suppressed BLT mice 48 h after the administration of vehicle control or AZD5582 (three control and one AZD5582-treated analysed). Positive cells, brown. Imaged at 4× and 40× magnifications; scale bars, 100 μm (4×) and 50 μm (40×). Boxes in the 4× images indicate regions corresponding to images at 40× magnification. For gel source data, see Supplementary Fig. 1.
