Extended Data Fig. 4: Speed characterization of translocation runs.
From: Processive extrusion of polypeptide loops by a Hsp100 disaggregase
a, Translocated length (Lt) during threading of 2MBP by ClpB(Y503D). Raw data (light grey, 500 Hz) is filtered using a Savitzky–Golay filter (black line). b, Local translocation speed calculated as the time derivative of the translocated length after Savitzky–Golay filtering. Negative slopes below −50 nm s−1 (horizontal line) are considered back-slipping events (orange areas, also in a) and help in determining isolated translocation runs. c, Identification of different speeds within a single translocation run. Linear fits are used to calculate the speed of the run (green and magenta lines), most times revealing two main velocities, one double that of the other. d, Time derivative of the filtered translocated length for a single run, with solid black lines indicating the threshold speeds to distinguish no translocation from single- and double-translocation velocities and green and magenta indicating the fitted velocity values (also shown in c).
