Extended Data Fig. 10: Beclin 1, UVRAG or ATG14 knockout does not affect levels of LKB1 protein or the phosphorylation of LKB1 at Ser428 induced by glucose starvation. | Nature

Extended Data Fig. 10: Beclin 1, UVRAG or ATG14 knockout does not affect levels of LKB1 protein or the phosphorylation of LKB1 at Ser428 induced by glucose starvation.

From: TLR9 and beclin 1 crosstalk regulates muscle AMPK activation in exercise

Extended Data Fig. 10

ac, Effects of beclin 1 (a), UVRAG (b) or ATG14 (c) knockout in U2OS cells on levels of LKB1 protein and phosphorylation of LKB1 at Ser428, 1 h after glucose starvation. Left, representative western blots of p-LKB1, total LKB1 and total AMPKβ in cells with indicated gene knockout (two independent gRNAs per target gene). Right, quantification of total LKB1/total AMPKβ (from four independent experiments) and p-LKB1/total LKB1 (from three independent experiments). Similar results were observed in each experiment. Data are mean ± s.e.m. Sample size is indicated in parentheses. Unpaired two-tailed t-test. NG, no gRNA. For uncropped gels, see Supplementary Fig. 1.

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