Extended Data Fig. 2: TLR9–beclin 1 interaction and AMPK phosphorylation increases in skeletal muscle, but not in spleen, during exercise.
From: TLR9 and beclin 1 crosstalk regulates muscle AMPK activation in exercise
a, Representative western blots of endogenous beclin 1 co-immunoprecipitated with endogenous TLR9–HA in vastus lateralis muscles from TLR9–HA mice and wild-type mice (negative control) at indicated durations of exercise. Asterisk denotes a nonspecific band also observed in wild-type mice. Similar results were observed in three independent experiments. b, Quantification of p-AMPKα(T172)/total AMPKα (representative blots are shown in Fig. 1b) in vastus lateralis muscles from TLR9–HA mice at indicated durations of exercise. Results are combined data from three independent experiments with similar results in each experiment. c, d, Representative blots (c) and quantification (d) of p-AMPKα(T172)/total AMPKα in the spleen of TLR9–HA mice at indicated durations of exercise. Results are combined data from two independent experiments with similar results in each experiment. e, f, Representative western blots (e) and quantification (f) of beclin 1 co-immunoprecipitated with TLR9–HA in spleens from TLR9–HA mice at indicated durations of exercise. Results are combined data from two independent experiments with similar results in each experiment. In b, d, f, data points are individual mice (sample size is indicated in parentheses). Data are mean ± s.e.m. Values at 0 min are considered to be 1. In b, d, f, unpaired two-tailed t-test with Hommel method. For uncropped gels, see Supplementary Fig. 1.
