Extended Data Fig. 6: Similar levels of adenine nucleotides, glycogen, total LKB1 and LKB1 phosphorylation at Ser428, and a similar response to an AMPK allosteric activator in wild-type and Tlr9−/− muscles.
From: TLR9 and beclin 1 crosstalk regulates muscle AMPK activation in exercise
a–e, Measurements of ATP (a), ADP (b), AMP (c), ADP/ATP ratio (d) and AMP/ATP ratio (e) in EDL muscles. f, Glycogen content in tibialis anterior muscles. g, h, Representative western blots (g) and quantification (h) of p-LKB1(S428) and total LKB1 in vastus lateralis muscles. Representative blots are from one experiment, and quantification data are combined from three independent experiments. Similar results were observed in each experiment. i, j, Representative western blots (i) and quantification (j) of AMPK activation markers (p-AMPK and p-TBC1D1) in tibialis anterior muscles of mice, 90 min after subcutaneous administration of PF-739 (100 mg per 5 ml, per kg body weight). k, Blood glucose levels 90 min after treatment with PF-739. In i–k, western blots are from one representative experiment, and quantification results are combined data from two independent experiments. Similar results were observed in each experiment. Data points are individual mice (sample size indicated in parentheses). Data are mean ± s.e.m. Unpaired two-tailed t-test. For uncropped gels, see Supplementary Fig. 1.
