Extended Data Table 1 Estimates of Km and kobs for substrate ubiquitylation

From: NEDD8 nucleates a multivalent cullin–RING–UBE2D ubiquitin ligation assembly

  1. S0 refers to unmodified substrate, S1 to substrate modified by a single ubiquitin and S2 to substrate modified with two ubiquitins. S0–S1 refers to the transition from unmodified substrate to Ub–substrate, and S1–S2 to the transition from Ub–substrate to Ub–Ub–substrate. Values for Km are the best-fit values derived from nonlinear regression in Prism, and value for kobs are the best-fit values derived from nonlinear regression in Mathematica. The measure of error is the standard error of the mean as determined by Prism and Mathematica, respectively, from experiments and curve fits such as those shown in Extended Data Fig. 1 (n = 2).
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