Extended Data Fig. 7: Kymographs of time-lapse experiments performed on LfngT2AVenus gastruloids that were embedded in 100% Matrigel at 96 h.

a–d, Kymographs (space–time plots) of bright field channel and LfngT2AVenus signal along the anterior–posterior axis of all replicates from all time-lapse experiments (experiments 1–4) that are presented in Fig. 2e, Extended Data Fig. 8e, f. These gastruloids were embedded in 100% Matrigel (Methods) to stabilize them during imaging, and subsequently imaged for at least 17 h (Supplementary Videos 1, 2, 4, 5). Inhibitors were added at the start of the time lapse (Methods) and are indicated above the kymographs, together with their concentration. Asterisks refer to gastruloids used to generate Fig. 2e, Extended Data Fig. 8b–d. Similar results were obtained in n = 5 and 4 independent experiments for a and b–d, respectively. e, Imaging of a LfngT2AVenus gastruloid that was embedded in 100% Matrigel at 96 h, and to which the Notch inhibitor DAPT was added at 96.5 h (Supplementary Video 2); the Lfng signal disappears about 6 h after addition of DAPT. Corresponding kymographs in a. Similar results were obtained in n = 5 independent experiments. Scale bar, 200 μm.