Extended Data Fig. 10: Uncx4.1 Tbx18 Ripply2 stainings and somite-size measurements.
From: Single-cell and spatial transcriptomics reveal somitogenesis in gastruloids
a, HCR21 double staining for Uncx4.1 (cyan) and Tbx18 (magenta) on 120-h LfngT2AVenus gastruloids embedded in 10% Matrigel at 96 h. For replicate 4, 1.3 μM of PD03 was added at 96.5 h. Similar results were obtained in n = 4 independent experiments. b, Similar to a, but for Uncx4.1 (cyan) and Ripply2 (yellow). Similar results were obtained in n = 2 independent experiments. c, Intensity of Uncx4.1 and Tbx18 signal along the anterior–posterior axis of the gastruloids in a. Peaks (circles) are called on the smoothened Uncx4.1 profile (dark blue) (Methods). d, Similar to c, but for the Uncx4.1- and Ripply2-stained gastruloids from b. e, Distance between Uncx4.1 peaks in the 120-h LfngT2AVenus gastruloids (n = 7) from replicates 1–6 in a–d and in replicate 7 (which is shown in Fig. 3c). Replicate 8 was excluded from quantification and both replicate 4 and replicate 7 were incubated in 1.3 μM PD03 from 96–120 h. Scale bars, 200 μm.
