Extended Data Fig. 3: Mapping-by-sequencing identification of mutations in HPCA1 in three allelic hpca1 mutants and their stomata responses to H2O2, ABA and external Ca2+.
From: Hydrogen peroxide sensor HPCA1 is an LRR receptor kinase in Arabidopsis
a−c, SNP concurrences across the five chromosomes are analysed and shown from a pooled mapping population of hpca1-1 (a), hpca1-2 (b) and hpca1-3 (c). All three mutants show an enrichment of SNP concurrences in the same area in the long arm of chromosome 5. Genes with SNPs in these mutants were compared, and three allelic mutations in the HPCA1 (At5g49760) gene were found as shown in Fig. 3a and Extended Data Fig. 6a. d, Images of wild-type and three allelic hpca1 mutants were taken after 30 or 60 days of growth under growth chamber conditions (16 h/8 h light/dark photoperiod). e−g, Stomatal apertures were measured in the absence or presence of 1 mM H2O2 (e), 5 μM ABA (f) or 2.5 mM CaCl2 (g). Data are mean ± s.e.m. from three independent experiments, n = 60 stomata. The stomatal apertures without the treatment in e were 3.38 μm in WT, 3.12 μm in hpca1-1, 3.41 μm in hpca1-2, and 3.26 μm in hpca1-3. The stomatal apertures without the treatment in f were 3.21 μm in WT, 3.37 μm in hpca1-1, 3.27 μm in hpca1-2, and 3.26 μm in hpca1-3. The stomatal apertures without the treatment in g were 4.36 μm in WT, 4.54 μm in hpca1-1, 4.40 μm in hpca1-2, and 4.55 μm in hpca1-3.
