Extended Data Fig. 8: Seeded tau aggregation induced by CBD filaments in SH-SY5Y cells. | Nature

Extended Data Fig. 8: Seeded tau aggregation induced by CBD filaments in SH-SY5Y cells.

From: Novel tau filament fold in corticobasal degeneration

Extended Data Fig. 8

a, Immunoblotting of sarkosyl-insoluble (Ppt) and sarkosyl-soluble (Sup) fractions extracted from mock-transfected SH-SY5Y cells and from cells transfected with tau seeds from the frontal cortex of CBD cases 1–3. SH-SY5Y cells transiently expressed either haemagglutinin (HA)-tagged 1N4R or HA-tagged 1N3R human tau. Insoluble tau was detected with anti-HA and anti-pS396 antibodies. Total tau was detected with anti-TauC. Blotting with an anti-α-tubulin antibody served as a loading control. b, Quantification of anti-HA-positive bands. The results are expressed as the mean ± s.e.m. (n = 3).

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