Extended Data Fig. 7: Genetic and chemical contributions that promote the attraction of D. sechellia to noni. | Nature

Extended Data Fig. 7: Genetic and chemical contributions that promote the attraction of D. sechellia to noni.

From: Olfactory receptor and circuit evolution promote host specialization

Extended Data Fig. 7

a, Data reproduced from Fig. 2d. Behavioural responses in a trap assay testing preference of the indicated genotypes for noni juice or grape juice. n = 13–25 experiments, 22–25 female flies per experiment. Comparisons to responses of Dsec.07 flies are shown. In ad, f, pairwise Wilcoxon rank-sum test and P values adjusted for multiple comparisons using the Benjamini and Hochberg method. Red bars, no significant difference; salmon bars, significantly different responses of D. sechellia genotypes. b, Proportion of flies (mean ± s.e.m.) in each stimulus trap for the assays shown in a. Comparisons to responses of Dsec.07 flies are shown. c, Olfactory responses in a trap assay testing preferences between noni juice and grape juice of wild-type D. sechellia, DsecOrco1 Ir8aGFP double mutants and wild-type D. sechellia with the third antennal segments removed (antennaless). n = 9–15 experiments, 22–25 female or male flies (as indicated) per experiment. These data represent the same experiments shown in Fig. 2e, but attraction indices were calculated here taking only alive flies into account. The percentages of flies alive at the end of the assay are indicated below, revealing the high mortality rate of antennaless flies and DsecOrco1 Ir8aGFP double mutants (DsecOrco2 Ir8aGFP mutants appeared to be nonviable). Normally, trap assay experiments with >25% fly mortality were discarded (Methods). Comparisons to responses of Dsec.07 flies are shown. d, Proportion of flies (mean ± s.e.m.) in each stimulus trap for the assays shown in c. Comparisons to responses of Dsec.07 flies are shown. e, Behavioural responses in a trap assay testing preferences between noni fruit and grape juice of Dsec.07 and DsecOr22aRFP flies. Comparisons to responses of Dsec.07 flies are shown. Pairwise Wilcoxon rank-sum test. f, Behavioural responses in a trap assay testing preferences between grape juice and 10−2 dilutions of the indicated odours in grape juice of D. sechellia, DsecOr22aRFP, D. simulans and D. melanogaster. Comparisons to responses of Dsec.07 flies to methyl hexanoate are shown. g, Behavioural responses in a wind tunnel assay testing attraction of D. sechellia to three single noni odours (10−2 dilution in water), a mix of all three in the approximate proportions of ripe noni fruit (1:0.04:1, methyl hexanoate:2-heptanone:hexanoic acid) and noni juice. n = 10 experiments, 10 female flies per experiment. Comparisons to responses of Dsec.07 flies to noni juice are shown. Kruskal–Wallis test with Dunn’s post hoc correction. NS, not significant (P > 0.05); *P < 0.05; **P < 0.01; ***P < 0.001.

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