Extended Data Fig. 12: Protein sequence alignments of Or22a and Ir75b.
From: Olfactory receptor and circuit evolution promote host specialization
a, Or22a orthologues of strains used for behavioural assays as well as genome-sequenced strains (‘gen.’) of each species (version: D. sechellia (r1.3), D. simulans (r2.01) and D. melanogaster (r6.28)). Blue or red shading indicates differences between species or strains, respectively. Green boxes, residues tested in this study for their role in defining ester tuning specificity. b, Ir75b orthologues of same strains as shown in a. Blue or red shading indicates differences between species or strains, respectively. Black boxes, residues predicted to be located within the ligand-binding domain that contributes to odour tuning specificity63. The premature stop codon of the Canton-S strain (position 169, marked by an asterisk) does not impair receptor function, as shown in other strains63.
