Fig. 7: Subcellular localization of RBPs and links to transcriptome binding and regulation.
From: A large-scale binding and functional map of human RNA-binding proteins
a, Examples of RBPs (green) co-localized with nine investigated markers (red). RBPs were imaged at five or more sites per co-labelling marker with twelve co-labelled markers in total, and representative images are shown. b, For localization patterns with known localized RNA classes, heatmap indicates significance (from one-sided Wilcoxon rank-sum test) comparing eCLIP relative information for the indicated RNA class (y-axis) for RBPs with versus without the indicated localization (x-axis). c, Bars indicate eCLIP relative information content (IP versus input) for mitochondria H-strand (grey) or L-strand (red). RBPs with mitochondrial localization in HepG2 cells are indicated in red. Inset shows immunofluorescence imaging for DHX30 (representative of ten sites imaged). d, Genome browser tracks indicate eCLIP relative information content along the mitochondrial genome (top) or a roughly 300-nt region for indicated RBPs (bottom). Inset shows RNA secondary structure prediction (RNAfold) for the indicated region. Tracks are shown for replicate 1; eCLIP and KD–RNA-seq were performed in biological duplicate with similar results.
