Extended Data Fig. 8: CPD deletion inhibits the IGF1R pathway in H322, A549 and H358 cells.
From: CRISPR screens in cancer spheroids identify 3D growth-specific vulnerabilities
a, Representative immunofluorescence images of IGF1R α-chain (green) in control and CPD-knockout H23 spheroids. b, Quantification of immunofluorescence in a. IGF1R α-chain intensities averaged across nine spheroids per condition. *P = 2.2 × 10−3 (n = 9, two-sided t-test; mean ± s.e.m.). c–e, IGF1R and phosphorylated AKT levels were quantified from immunofluorecence images for H322 (c), A549 (d) and H358 (e) cells. The dotted grey line marks a 100% level (P values calculated using two-sided t-test; mean ± s.e.m.).
