Extended Data Fig. 6: Pharmacological properties of spine plasticity in D2-SPNs.
From: Dopamine D2 receptors in discrimination learning and spine enlargement
a, Example trace of 2pEPSC (average of five traces with stimulation of four spines from one slice). b, Membrane potential during one train of stimulation (top) and command of uncaging (red) and current injection (black) (bottom). To mimic the up state, constant current (~200 pA) was injected for 1.1 s and APs were evoked by pulsed current injections (0.6–2.0 nA, 2 ms). c, Magnified traces of membrane potentials. Note the 2pEPSP, right. d, Schematic of virus injection to label D2-SPNs e, Schematic of stimulation and representative images of dendritic spines in mCherry-expressing D2-SPNs. Red circles show two-photon uncaging spots. f, g, Time course of volume changes of stimulated and neighbouring spines in the absence of CGS (f) and in the absence of CGS with sulpiride (g). STDP stimulation was applied at 0 min. n = 5 (f) and 6 slices (g). h, Viral expression of AIP in D2-SPNs. i, Schematics of STDP stimulation of AIP-expressing D2-SPNs in the presence of CGS (top) and representative images of dendritic spines (bottom). j, Time courses of volume changes of stimulated and neighbouring spines in AIP-expressing D2-SPNs. A reference trace of mCherry is the same as in Fig. 3d. n = 6 slices. k–m, Electrophysiological properties of D2-SPNs expressing AIP. n = 10 and 6 slices for mCherry and AIP. Plots of resting membrane potential (RMP) (k), two-sided Mann–Whitney U test (U = 22, P = 0.39); 2pEPSC (l, right), two-sided Mann–Whitney U test (U = 23, P = 0.45); averaged traces of 2pEPSC (l, left); and membrane potentials during STDP stimulation (m) are shown. n–q, Spine plasticity of D2-SPNs in the presence of CGS with PKI (o, p, n = 6 slices) or AP5 (q, n = 6 slices). r, A schematic of virus injection to label D1-SPNs using Cre-off mCherry-expressing AAV (FAS-NLS-mCherry)44. s, Representative images of dendritic spines stimulated in the presence of CGS21680 (top) or puff-applied DA (bottom) in D1-SPNs. t, Time course of volume changes. n = 6 for CGS and 5 slices for DA (puff). u, Plots of volume changes. n = 6 for CGS, n = 5 slices for DA (puff). Two-sided Mann–Whitney U test (U = 0, **P = 0.006). Scale bars in e, i, o, s indicate 2 μm. Error bars indicate s.e.m. Samples of slices are biological replicates.
