Extended Data Fig. 7: Single-particle cryo-EM characterization of MthK Ca²⁺-bound states.

a–d, MthK Ca²⁺ state 1 (highly tilted). a, Final cryo-EM map coloured by local resolution. b, FSC curve between two independently refined half-maps, after masking. c, Selected 2D-class averages. d, Angular distribution of particles used in the reconstitution. e–h, MthK Ca²⁺ state 2 (medium-tilted) e, Final cryo-EM map coloured by local resolution. f, FSC curve between two independently refined half-maps, after masking. g, Selected 2D-class averages. h, Angular distribution of particles used in the reconstitution. i–l, MthK Ca²⁺ state 3 (mildly tilted). i, Final cryo-EM map coloured by local resolution. j, FSC curve between two independently refined half-maps, after masking. k, Selected 2D-class averages. l, Angular distribution of particles used in the reconstitution. m–p, Ca²⁺-bound closed MthK. m, Final cryo-EM map coloured by local resolution. n, FSC curve between two independently refined half-maps, after masking. o, Selected 2D-class averages from particles. p, Angular distribution of particles used in the reconstitution.