Extended Data Fig. 1: The purification of SARS-CoV-2 Mpro and the inhibitory assay of the N3 compound.
From: Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors
a, SDS–PAGE gel of SARS-CoV-2 Mpro. First lane, marker; second lane, Mpro before treating with rhinovirus 3C protease; third lane, Mpro after the cleavage of C-terminal His tag. For gel source data, see Supplementary Fig. 1. b, Size-exclusion chromatography profile of Mpro. c, The chemical structure of the N3 inhibitor. d, Inhibition mechanism for N3. e, Typical inhibition curves for N3. f, Cytotoxicity assay of N3 on Vero cells. Data are shown as mean ± s.e.m., n = 3 biological replicates. The data in a, b, e, f are representative of three independent experiments with similar results.
