Extended Data Fig. 9: Fluidigm-based transcriptional analysis of Gfi1R412X/− genetic rescues.
From: Mouse models of neutropenia reveal progenitor-stage-specific defects
a, b, FACS plots (a) and representative cytospins (b) of bone marrow from adult mouse. c, d, Graphical summary of CFU assays performed on KIT+ bone marrow cells. e, FACS plots of mouse bone marrow cells isolated from Irf8-eGFP transgenic mice with the mean percentage of IRF8–GFPhigh cells indicated. f, Three-dimensional FACS plots of bone marrow isolated from adult Irf8-eGFP mice. Events are pseudocoloured for CD11b expression and arrows indicate changes in the incidence of granulocytic populations. g, FACS plots and representative cytospins of adult mouse bone marrow populations sorted for scRNA-seq. h, i, cellHarmony-assigned Gfi1R412X/R412X and Gfi1R412X/−Irf8+/− cells to wild-type reference Fluidigm scRNA-seq populations (h, n = 86 cells, i, n = 88 cells). Each tick mark represents a single-cell library. ICGS clusters are annotated (top). FACS gates are annotated (right). j, Population distribution of Fluidigm scRNA-seq data from h, i and Extended Data Figs. 4c, 6c. j, k, Fluidigm scRNA-seq heat maps (left) of genes that are genetically repaired in each cluster, with key genes indicated. Adjacent plot (right) of enriched biological processes in the indicated clusters. Scale bars, 10 μm. Cytospin data in b are representative of three biological replicates. Data are mean ± s.e.m. (a, c, d) or mean (e). ****P < 0.0001, two-tailed t-test.
