Extended Data Fig. 2: AVE migration regulates the distribution of basement membrane perforations.
From: Basement membrane remodelling regulates mouse embryogenesis

a, AVE position and basement membrane remodelling using transgenic Cerberus–GFP embryos for AVE identification. n = 23 embryos. b, Quantification of basement membrane area covered by perforations at regions away (proximal or posterior) or close to the AVE side as in Fig. 2b, with embryos from different AVE migration stages pooled together. Two-sided unpaired Student’s t-test; ****P < 0.0001. In box plots, centre lines show median values, box limits represent the upper and lower quartiles, and whiskers show the range of values. c, Representative examples of E6.5 pre-gastrula control (n = 20 embryos) and Ttr-cre;Rhoafl/− embryos (n = 7 embryos). Blocking AVE migration results in abnormal distribution of basement membrane perforations. d, Representative examples of control (n = 5) and Ttr-cre;Rhoafl/− (n = 5) E5.5 embryos. Scale bars, 20 μm.