Extended Data Fig. 4: MMP expression profiles.
From: Basement membrane remodelling regulates mouse embryogenesis

a, UMAP showing distribution of cells by embryonic age. n = 1,724 cells; n = 331 (E5.25), 269 (E5.5), 321 (E6.25), 803 (E6.5) cells. b, Marker genes identify epiblast (Pou5f1), extraembryonic ectoderm (Bmp4) and visceral endoderm (Amn) cells in the UMAP. n = 775 epiblast cells, 283 extraembryonic ectoderm cells and 666 visceral endoderm cells. c, Expression levels of MMP genes on UMAP plot for E5.25–E6.5. Expression values are log(counts). Blue box, epiblast. d, mRNA levels of Cerberus and Mmp25 at the anterior and posterior side of the embryo. Anterior and posterior identity was defined based on Cerberus expression. Mmp25 is highly expressed at the anterior side of the embryo. n = 3 embryos. Two-sided unpaired Student’s t-test. Cerberus: **P = 0.001; Mmp25: ***P = 0.0002; mean ± s.e.m. e, E5.5 and E6.5 pre-gastrula embryos stained for MMP14. White arrowheads, prospective primitive streak. n = 10 embryos for each stage. Scale bars, 20 μm. f, The matrix of reads per kilobase of transcript per million mapped reads (RPKM) from GSE109071 was used to calculate gene-expression correlation between pairs of genes. Epiblast cells at E6.25 and E6.5 (n = 527 cells) were extracted from the matrix and Pearson's correlation coefficient (R) with two-sided P value are given for each comparison. The points are coloured by density (kde2d).