Extended Data Fig. 6: crRNA performance during HV panel testing.
From: Massively multiplexed nucleic acid detection with Cas13
a, Individual guide performance in rounds 1 and 2. Redesign and re-dilution between rounds of testing are indicated between the data from rounds 1 and 2. On-target: reactivity above threshold for intended target only. Cross-reactive: off-target reactivity above threshold. Low activity: no reactivity above threshold. b, Summary bar graph of crRNA performance in rounds 1 and 2. c, Summary table of redesign, re-dilution and concordance between rounds 1 and 2 for unchanged tests. d, e, Round 1 (d) and round 2 (e) ranked AUCs for ROCs for on-target versus off-target reactivity in round 1 of testing. Representative on-target and off-target distributions are shown for the indicated ranks.
