Fig. 3: Influenza subtype discrimination with CARMEN–Cas13.
From: Massively multiplexed nucleic acid detection with Cas13
a, Schematic of influenza A subtype discrimination using CARMEN–Cas13. b, Discrimination of H1–H16 using CARMEN–Cas13. c, Discrimination of N1–N9 using CARMEN–Cas13. d, Identification of H and N subtypes from patient samples and mixed synthetic targets (Methods, ‘Cas13 detection reactions’ under ‘General procedures’). Heat maps indicate background-subtracted fluorescence after 1 h (for H1–H16 discrimination) or 3 h (for N1–N9 discrimination) of Cas13 detection. In b–d, synthetic targets were used at 104 copies per μl. Asterisks in d indicate a signal above threshold for a synthetic target or patient sample.
