Extended Data Fig. 7: Filamentous α-synuclein pathology in DLB.
From: Structures of α-synuclein filaments from multiple system atrophy
a, Staining of inclusions in the frontal cortex in DLB cases 1 and 2 and the amygdala in DLB case 3 by the antibody pS129 (brown). Scale bar, 50 μm. b, Negative-stain electron microscopy images of filaments from the frontal cortex in DLB cases 1 and 2 and the amygdala in DLB case 3. Scale bar, 50 nm. c, Representative immunogold negative-stain electron microscopy images of α-synuclein filaments extracted from the frontal cortex in DLB cases 1 and 2 and the amygdala in DLB case 3. Filaments were labelled with the antibody PER4, which recognizes the C terminus of α-synuclein. Arrowheads point to an unlabelled tau paired helical filament. Scale bar, 200 nm. d, Representative cryo-EM images of α-synuclein filaments from the frontal cortex in DLB cases 1 and 2, and the amygdala in DLB case 3. Scale bar, 200 nm. Arrowheads point to a tau paired helical filament, as evidenced by a three-dimensional reconstruction (inset), calculated as previously described6. e, Two-dimensional class averages of α-synuclein filaments extracted from the frontal cortex in DLB cases 1 and 2 and the amygdala in DLB case 3.
