Nature

Table 1 RT–qPCR testing results on nasal and oral swabs of the dogs and serology

From: Infection of dogs with SARS-CoV-2

		TLVL laboratory				HKU laboratory
		C_t (E)		C_t (RdRp)		C_t (nsp14)		C_t (N)			C_t (nsp16)		C_t (M)		Serum PRNT₉₀ titre
	Date of collection	Nasal	Oral	Nasal	Oral	Nasal	Oral	Nasal	N gene copies per ml (nasal)	Oral	Nasal	Oral	Nasal	Oral
Dog 1 (potential exposure 12–26 Feb)	26 Feb	33.90	34.52	38.97	Neg.	36.76	37.96	34.71	11,741	36.48	37.94	39.25	36.91	37.95
	28 Feb	31.98	Neg.	37.44	Neg.	38.96	39.01	34.58	10,145	Neg.	38.64	Neg.	38.97	Neg.
	2 Mar	31.69	Neg.	Neg.	Neg.	32.49	Neg.	33.2	25,788	Neg.	32.71	Neg.	32.41	Neg.
	3 Mar														1:80
	5 Mar	33.58	Neg.	38.53	Neg.	39.14	Neg.	38.43	751	Neg.	37.72	Neg.	Neg.	Neg.
	9 Mar	30.07	Neg.	Neg.	Neg.	35.86	Neg.	34.97	7,777	Neg.	36.96	Neg.	36.24	Neg.
	12 Mar	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.
	13 Mar	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.
Dog 2 (potential exposure 10–17 Mar)	18 Mar	24.85	26.60	31.19	32.63	26.74	28.72	27.31	724,500	29.33	28.26	30.29	27.73	29.49
	19 Mar	28.11	31.23	36.12	38.45	32.98	36.09	32.66	62,933	36.98	33.65	36.95	32.17	35.97	<1:10
	20 Mar	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.
	23 Mar	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	1:40
	24 Mar	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.
	30 Mar	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	Neg.	1:160
Cut-off C_t for positive		<36	<36	<39	<39	<40	<40	<40	<40	<40	<40	<40	<40	<40

The E, nsp14 and N RT–qPCR assays are cross-reactive with SARS-CoV-2, SARS-CoV and closely related bat-SARS CoV viruses. The RdRp, nsp16 and M RT–qPCR assays are specific for SARS-CoV-2. Gene copies per millilitre of original swab specimen with adjustment for virus extraction dilutions. Assumes no pre-symptomatic shedding of virus from human cases. Neg., negative.

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