Extended Data Fig. 2: Mg2+ titration of the miR-34a–mSirt1 bulge followed by NMR.
From: Base-pair conformational switch modulates miR-34a targeting of Sirt1 mRNA
Shown are HSQC overlays of different Mg2+ titration steps. a, 1H–13C aromatic 2/6/8 HSQC. b, 1H–13C sugar 1′ HSQC. c, 1H–15N imino 1/3 HSQC. The titration steps are colour-coded (a, top left). Additional overlay of the miR-34a–mSirt1 trapped ES is shown in grey in a, b. Arrows indicate the chemical-shift trajectory during titration. Dashed lines connect equivalent peaks in the miR-34a–mSirt1 bulge and trapped ES constructs.
