Extended Data Fig. 1: The effects of LbNOX expression in primary hepatocytes.
From: Hepatic NADH reductive stress underlies common variation in metabolic traits
a, Dose-dependent adenovirus-mediated expression of LbNOX in primary hepatocytes at 24 h. A representative western blot from two independent experiments is shown. b, Effect on basal or antimycin + rotenone-insensitive respiration with LbNOX or mitoLbNOX. n = 3. c, Effect of LbNOX on free cytosolic NADH/NAD+ as measured by Peredox with increasing alcohol concentrations. d–g, Whole cell NAD+ (d), NADH (e), NADH/NAD+ (f) and NADH + NAD+ (g). n = 6 independent hepatocyte isolations. Nominal P values were determined using paired, two-sided Student’s t-tests between hepatocyte isolations (b, d–g) or unpaired, two-sided Student’s t-test for Peredox experiments (c). Data are mean ± s.e.m.
