Extended Data Fig. 10: Hair follicles of xenografted WA25 skin organoids have bulge regions comparable to second-trimester (18 weeks) fetal and adult facial hair.
From: Hair-bearing human skin generated entirely from pluripotent stem cells
a–d, Representative immunostaining images for markers of hair follicle stem cells (LHX2, KRT15 and NFATC1) in the hair follicle bulge region in 18-week human fetal skin from two facial locations (forehead and cheek) (a, b), adult facial skin (c) and xenografted skin organoid tissue (d). In both fetal and xenograft hair follicles, NFATC1 expression is predominantly localized to the cytoplasm in bulge cells, whereas in adult facial hair follicles, NFATC1 expression is nuclear-localized in the hair follicle bulge region—consistent with previous reports of nuclear-localized NFATC1 in mouse bulge stem cells36. Arrows indicate background (BG) staining noises. Dashed boxes in a, c, d indicate magnified bulge regions, which are presented in a corner of each image. Dashed brackets in b indicate the bulge region. Representative immunostaining images are selected from five independent stainings on three to five different samples per tissue type. Scale bars, 100 μm (a, c); 50 μm (b, d). This figure corresponds with the data in Fig. 4.
