Extended Data Fig. 3: Comparison of initial hair follicle induction in WA25 and DSP-GFP live-cell aggregates.
From: Hair-bearing human skin generated entirely from pluripotent stem cells
a, Representative DIC images of WA25 skin organoids at days 65–130 with developing hair follicles. Scale bars, 500 μm (left); 250 μm (right). b, DIC and endogenous GFP fluorescence images of DSP-GFP skin organoids at days 65–135 with developing hair follicles. Dashed boxes indicate magnified regions (right). Scale bars, 500 μm (left, middle); 250 μm (far right, days 95, 110 and 135); 100 μm (far right, days 65 and 85). c, DIC images of WA01 skin organoids at days 68–98 with developing hair follicles. Right, magnified view of day-88 WA01 skin organoid hair follicles. The head (skin cyst) and tail (non-skin mesenchymal) portions within skin organoids are distinguishable. In addition, the hair bulbs are facing outward from the organoid, contacting the medium, whereas the hair shafts grow inward towards the centre of the skin organoid. Scale bars, 250 μm (all panels except far right); 50 μm (far right). The WA25 and DSP-GFP skin organoid images represent morphologies observed throughout nine independent cultures, and the WA01 skin organoid images represent one experiment performed by the Stanford group. This figure corresponds with the data in Fig. 1e, f.
