Extended Data Fig. 8: CDK inhibitors block the CR8-induced degradation of cyclin K.
From: The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K
a, Titration of CDK12–cyclin KAlexa488 into DDB1terbium in the presence of the indicated compounds (all 10 μM) (n = 3). b, NanoBRET of HEK293T cells transfected with NanoLuc-labelled CDK12(713–1052) and HaloTag-labelled DDB1(∆BPB) constructs and treated with the indicated compounds for 2 h. Bars represent the mean (n = 3). c, Flow analysis of cyclin KeGFP degradation in HEK293T-Cas9 cells treated with 1 μM CR8 and competitive CDK inhibitor (n = 3).
