Extended Data Fig. 9: Cytotoxicity of CR8 analogues does not depend on CRL4 components.
From: The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K
a, Drug sensitivity of HEK293T-Cas9 cells that were treated with different inhibitors for three days (n = 3). b, Drug sensitivity of HEK293T-Cas9 cells that were treated with 100 nM MLN4924 or DMSO in combination with the indicated compound for three days (n = 3). c, Immunoblots of HEK293T-Cas9 cells transfected with control (pRSF91-GFP) or CRBN overexpression (pRSF91-CRBN) vectors (n = 2). d, Immunoblots of HEK293T-Cas9 cells expressing pRSF91-GFP or pRSF91-CRBN and treated with CR8 for three days (n = 2). e, Drug sensitivity of HEK293T-Cas9 cells expressing pRSF91-GFP or pRSF91-CRBN and treated with CR8 for three days (n = 3). f, Immunoblots of HEK293T-Cas9 cells transfected with control (pLX307-Luc) or CRBN overexpression (pLX307-CRBN) vectors (n = 2). g, Flow analysis of cyclin KeGFP degradation in HEK293T-Cas9 cells expressing pLX307-Luc or pLX307-CRBN and treated with CR8 for 2 h (n = 3). h, Drug sensitivity of HEK293T-Cas9 cells expressing sgRNAs targeting DDB1 or luciferase and treated with the indicated inhibitor for three days (n = 3).
