Extended Data Fig. 2: Interlaboratory reproducibility and prediction of peptide retention time on the μPAC column.
a, The ability to produce chip-based columns in a reproducible manner, coupled with the statically fixed micrometre-sized pillars, results in highly reproducible performance and interlaboratory transferability of the μPAC-based approach. Shown are the corrected retention times of an excerpt of 5,000 peptides from the 43,000 overlapping peptides measured in two different HeLa cell digests by our Munich and Copenhagen laboratories, resulting in a Pearson correlation coefficient of peptide retention times of 0.995. b, To validate our model for predicting peptide retention times, we plot an excerpt of 1,000 peptides from the complete test-set of 54,490 peptides, with experimentally determined values on the x axis and predicted values on the y axis. The Pearson’s R2 correlation value for the complete predicted peptide set is 0.99.
