Extended Data Fig. 6: β-Catenin acetylation depends on pHi and glycolytic activity.
From: Intracellular pH controls WNT downstream of glycolysis in amniote embryos
a, b, Western blot analysis using anti-acetylated K49 β-catenin, anti-active β-catenin, anti-actin and anti-β-catenin. Extracts of 2-day-old chicken embryos cultured in minimal medium with 8.3 mM glucose at various pH (n = 3 per condition) (a) or in minimal medium at pH 7.2 with various glucose concentrations (n = 4 per condition) (b). c, Quantification of acetylated lysine and β-catenin intensity in Fig. 3h using Fiji. The acetylation rate is calculated from the slope of the graph. n = 2 independent experiments. Linear approximation, mean ± s.d. of the slope. For gel source data, see Supplementary Fig. 1.
