Extended Data Fig. 5: Replication of TCR with lesion strand resolution in Mus musculus castaneus.
From: Pervasive lesion segregation shapes cancer genome evolution
a, TCR of template strand lesions is dependent on transcription level (P15 liver, median TPM). Mutation rate estimates (circles) are the aggregate rates for expression level binned genes across CAST tumours (n = 84). Expression level bin 0 contains n = 2,645 genes, all subsequent bins contain n = 4,323 genes. See Methods for per-gene, per-tumour inclusion criteria. Empiric confidence intervals (99%) were calculated through bootstrap sampling (n = 100 replicates) of genes within the expression level bin. b, Comparison of mutation rates for the 64 trinucleotide contexts: each context has a high and a low expression point linked by a line. c, Sequence composition normalized profiles of mutation rate around TSS loci. d, Stratifying the data plotted in c by lesion strand reveals greater detail on the observed mutation patterns, including the pronounced influence of bidirectional transcription initiation.
