Extended Data Fig. 1: Pharmacology and cryo-EM data collection and processing for TRPA1.
From: Irritant-evoked activation and calcium modulation of the TRPA1 receptor
a, All points histograms depicting the change in open probability (P(o)) in a single TRPA1 channel in response to IA application. Data represent n = 9 independent excised inside-out patches. Vhold = −40 mV. b, SDS–PAGE showing MBP–TRPA1 (arrowhead) after pull-down and elution from amylose beads. c, Cryo-EM image of MBP–TRPA1. Scale bar, 20 nm. d, Two-dimensional classification of cryo-EM particle images showing TRPA1 in different orientations. Scale bar, 25 Å. e, Pharmacological agents used in this study.
