Extended Data Fig. 7: Treatment with antibiotics unleashes tumour-suppressive effects of mutant p53 in the distal gut.
From: The gut microbiome switches mutant p53 from tumour-suppressive to oncogenic
a, RT–qPCR of the 16S subunit of microbial rRNA in the ileum of vehicle- or antibiotic-treated mice. Mean ± s.e.m. (n, number of mice) relative to vehicle-treated CKIafl/fl (normalized to 1), one-sided Student’s t-test. The y axis is represented as log10-transformed expression. b, Length of crypts in mouse ileum. Overall average of the mean values for each mouse ± s.e.m (n, number of crypts), one-sided Student’s t-test. c, d, g, H&E-stained sections (c) and IHC of Ki67 (d) and WNT targets (g) in mouse colon. Scale bars, 100 μm. e, IHC of WNT targets in mouse ileum. Scale bars, 100 μm. f, RT–qPCR of WNT targets in ileal enterocytes from vehicle- or antibiotic-treated mice. Mean ± s.e.m. (n, number of mice) relative to vehicle-treated CKIafl/fl mice (normalized to 1), one-sided Student’s t-test. Representative data from four independent experiments (c, d, e, g).
