Extended Data Fig. 2: Mutant p53 exerts oncogenic GOF at the distal gut and tumour-suppressive effects at the proximal gut.
From: The gut microbiome switches mutant p53 from tumour-suppressive to oncogenic
a, H&E-stained sections and IHC of CKIα and p53 in mouse ileum and colon, three days after knockout induction. Inserts (H&E) show high-magnification images of colon and ileum, demonstrating the grade of dysplasia. Scale bars, 100 μm. b, Colon thickness of mice described in (a). Overall average of the mean values for each mouse ± s.e.m (n, number of colon thickness measurements), one-sided Student’s t-test. c, H&E-stained sections and IHC of p53, PROX1 and Ki67 in indicated mouse gut segments. Inserts (H&E) show high-magnification images of jejunal villi, demonstrating the grade of dysplasia. Scale bars, 100 μm. d, RT–qPCR of p53 targets in mouse jejunal enterocytes indicating that mutant p53 has not regained wild-type transcriptional activity. Mean ± s.e.m. (n, number of mice) relative to CKIafl/fl (normalized to 1), one-way ANOVA with Tukey’s test. e, IHC of p21 in indicated mouse gut segments. Scale bars, 100 μm. Representative data from two (a) or six (c, e) independent experiments.
