Extended Data Fig. 4: Mutant p53 suppresses the expression of WNT targets exclusively in the proximal gut.
From: The gut microbiome switches mutant p53 from tumour-suppressive to oncogenic
a, IHC of WNT targets in mouse jejunum. Scale bars, 100 μm. b, c, RT–qPCR of WNT targets in mouse jejunal (b) and ileal (c) enterocytes. Mean ± s.e.m. (n, number of mice) relative to CKIafl/fl (normalized to 1), one-way ANOVA with Tukey’s test. d, e, IHC of WNT targets in mouse ileum (d) and colon (e). Scale bars, 100 μm. f, g, Over-representation analysis of enriched gene sets differentially expressed in jejunum (f) and ileum (g) of CKIaΔgutp53R172H versus CKIaΔgutp53Δgut mice; negative logarithm of Benjamini–Hochberg-corrected P values (data derived from three mice of each group). Red lines indicate the significance threshold for α = 0.05. Representative data from four independent experiments (a, d, e).
