Extended Data Fig. 5: Mapping of critical contact residues for monoclonal antibodies by alanine and arginine mutagenesis and biolayer interferometry.
From: Potently neutralizing and protective human antibodies against SARS-CoV-2
a, Bar graphs show response values for monoclonal antibody binding to wild-type or mutant SRBD constructs normalized to the wild type. Asterisks denote residues where increased dissociation of monoclonal antibody was observed, probably indicating that the residue is proximal to the monoclonal antibody epitope. Full response curves for monoclonal antibody association and dissociation with wild-type or mutant SRBD constructs are also shown. b, Structure of the RBD, highlighting the critical contact residues for several monoclonal antibodies and their location on the structure.
