Extended Data Fig. 6: A positive impact of reduced CTCF levels on RAG scanning across the VH locus.
From: CTCF orchestrates long-range cohesin-driven V(D)J recombinational scanning
a–h, HTGTS-V(D)J-seq analysis showing average utilization frequencies ± s.d. of VH segments across four highlighted VH domains in RAG2-complemented and G1-arrested parental (a, b), OsTir1-expressing (c, d), CTCF-tagged (e, f), and CTCF-degron (g, h) cells without or with IAA treatment. n=4 (a–f) or n=6 (g, h) biologically independent repeats with similar results. Utilization of VHs presented in b–h are compared to those in NT parental cells shown in a, with red, blue, and grey bars indicating VHs with significantly increased, decreased, and unchanged utilization, respectively. To facilitate direct comparisons, the HTGTS-V(D)J-seq data for the IAA-treated parental and CTCF-degron cells shown in b and h are the same as those presented in Fig. 3b, c, respectively, with different y-axis scales. p values were calculated using unpaired two-tailed t-test (*: p ≤ 0.05, **: p ≤ 0.01, and ***: p ≤ 0.001). See Supplementary Table 3 for exact p values. See Methods for details.
