Extended Data Fig. 6: PbgA mutants and outer membrane permeability.
From: Structure of the essential inner membrane lipopolysaccharide–PbgA complex
a, All UPEC-ΔpbgA bacteria tested in the rifampicin sensitivity assay were probed by western blot analysis to confirm PbgA–Flag expression. GroEL was assessed as a loading control. Representative blots for n = 3 or more experiments are shown. b, Outer membrane permeability of UPEC ΔpbgA strains with pBADpbgA plasmids expressing wild-type or mutant pbgA assessed by rifampicin sensitivity, where MTR-AVA is the M212A/T213V/R216A PbgA triple mutant. Data are representative and presented as mean ± s.d. for n = 3 or more independent cultures. Note, see Extended Data Fig. 2f for a view of the salt-bridge interaction between R215 (IFD) and a conserved TMD acidic residue, D192.
