Extended Data Fig. 7: Co-expression and pulldown validation of C9orf72–SMCR8 and SMCR8–WDR41 interface.
From: Structure of the C9orf72 ARF GAP complex that is haploinsufficient in ALS and FTD
a, Close-up view of the residues that mediate the DENN– DENN dimerization between C9orf72 and SMCR8. b, Co-expression and pulldown experiment of Strep-tagged SMCR8 with GST–C9orf72 mutants and WDR41. c, Pulldown experiment of GST–WDR41 mutants with C9orf72–SMCR8. The pulldown experiments were carried out at least twice with similar results (b, c).
