Extended Data Fig. 1: Purification of the C9orf72–SMCR8–WDR41 and C9orf72–SMCR8 complex, as well as the HDX data for the trimer.
From: Structure of the C9orf72 ARF GAP complex that is haploinsufficient in ALS and FTD
a, The Superose-6 gel filtration elution profile for the C9orf72–SMCR8–WDR41 complex. b, The Superose-6 gel filtration elution profile for the C9orf72–SMCR8 complex. mAU, milli-absorbance units. c, The purified full-length C9orf72–SMCR8–WDR41 and C9orf72–SMCR8 complexes were analysed by SDS–PAGE. The proteins were purified at least five times with similar results (a–c). d–f, Deuterium uptake data for the C9orf72–SMCR8–WDR41 complex at the 0.5-s time point, with error bars from triplicate technical measurements. Peptides with more than 50% deuterium uptake are the flexible regions. Y axis represents the average per cent deuteration. X axis demonstrates the midpoint of a single peptic peptide.
