Extended Data Fig. 3: Contribution of non-essential RNAP subunits and transcription factors to fast transcription.
From: Functionally uncoupled transcription–translation in Bacillus subtilis
Induction time course of pycA-lacZα mRNA in various mutant backgrounds as in Fig. 1b, d. Time course of the same construct in WT from Fig. 1d also shown for reference. Lines indicate linear fits after signals rise. Uncertainties are standard error of the mean (s.e.m) among biological replicates (1 for ∆ykzG and 2 for all others). Time of appearance of full-length mRNA in mutants is not substantially different than that measured in WT (Supplementary Discussion SN4).
