Extended Data Fig. 7: Model Holliday junctions are not incised by MutLγ.
From: PCNA activates the MutLγ endonuclease to promote meiotic crossing over
a, b, Representative gel images of ensemble endonuclease reactions with Holliday junction (HJ) and nicked Holliday junction (nHJ) substrates. Reactions contained 25 nM indicated proteins, 0.5 mM ATP and 5 mM Mg2+/Mn2+, and were incubated at 37 °C for 30 min. The reaction products were analysed on a 15% denaturing polyacrylamide gel to detect strand nicking at any position. Nicking was not detected consistent with non-denaturing gels showing that model Holliday junction and nicked Holliday junction structures are not resolved by the MutLγ ensemble (not shown).
