Extended Data Fig. 4: Colocalization of MutLγ and MutSγ in mouse spermatocytes.
From: PCNA activates the MutLγ endonuclease to promote meiotic crossing over
a, Representative images of surface-spread spermatocyte chromosomes from the indicated pachytene substages, immuno-stained for MSH4 (green), MLH1 (magenta) and the chromosome axis marker SYCP3 (blue). Magnified panels show individual pairs of synapsed chromosomes. Scale bars, 10 and 2 μm, respectively. Arrowheads indicate crossover-specific MLH1 foci that colocalize with MSH4. b, Quantification of MSH4 and MLH1 foci and MSH4–MLH1 cofoci in early, mid and late-pachytene stages (n = 12, 15 and 13 nuclei, respectively). Error bars indicate mean ± s.e.m. N/A, not applicable because MLH1 foci were not observed in early pachytene. c, Percentage of MSH4 foci that are colocalized with MLH1 in mid and late pachytene nuclei (n = 12, 15 and 13 nuclei, respectively). Error bars indicate mean ± s.e.m. The percentage of co-foci increases as most MSH4 foci disappear in late pachynema.
