Extended Data Fig. 2: Morphology of SARS-CoV-2 virions released from infected Calu-3 cells.
From: Structures and distributions of SARS-CoV-2 spike proteins on intact virions
As in Extended Data Fig. 1. a, Histogram of virion diameters. Mean and standard deviation for diameters are 104 ± 13 nm (n = 67). b, Scatter plot of number of spikes identified per virion during subtomogram averaging against virion diameter for the same virions shown in a. c, Central slices through three representative viruses from 67 imaged in one experiment. Virions from Calu-3 cells had a slightly broader diameter distribution than those from Vero E6 cells. Scale bar 50 nm. d, Western blot analysis of SARS-CoV-2 nsp3, S and N in cell lysates and in virus preparations. In released virions, S is present in both cleaved (S2, 73%) and uncleaved forms (S0, 27%).
