Extended Data Fig. 9: Violet light deprivation alters BAT innervation and sensitivity to sympathetic nervous system input.
From: Violet-light suppression of thermogenesis by opsin 5 hypothalamic neurons
a, Lighting protocol used to generate ‘full spectrum’ and ‘minus violet’ mice. b, Spectral quality of lighting used in ‘full spectrum’ (top) and ‘minus violet’ (bottom) housing. Colored boxes indicate wavelength bounds used to estimate flux (photons cm-2s-1). c, UCP1 IHC of ‘full spectrum’ (top) and ‘minus violet’ (bottom) mice. d, Immunoblots of UCP1 at baseline (22 °C) and following 72 h cold adaptation (72h 4 °C) between ‘full spectrum’ (n = 3) and ‘minus violet’ (n = 3) mice. e–g, Representative images (e) of TH+ (tyrosine hydroxylase) innervation of BAT used for quantification represented in (f) and (g). h, Core temperature assessment (rectal) of ‘full spectrum’ and ‘minus violet’ mice during a 3h cold challenge. i, QPCR of thermogenesis genes (Ucp1, Pgc1α, Prdm16, Cidea) in iBAT from the mice used in (h). j, Adipose depot weight (mg) comparison between ‘full spectrum’ (n = 5) and ‘minus violet’ (n = 5) mice. k, Representative images highlighting inWAT cell size (haematoxylin and eosin) and iWAT UCP1 (IHC). l, Quantification of inWAT cell size H&E images for ‘full spectrum’ (n = 4) and ‘minus violet’ (n = 4) groups. m, Indirect calorimetry from ‘full spectrum’ (n = 6, grey trace) and ‘minus violet’ (n = 6, purple trace) mice treated with 1.0 mg kg−1 β3 adrenergic receptor agonist CL-316,243 (solid line) or vehicle (saline, dotted line). Administration of agonist or saline was performed at the 1 h time point (indicated by arrow). Data are mean ± s.e.m. P values are from one-way repeated measures ANOVA (f, h, l, m), ANOVA with Tukey post hoc analysis (i), or two-tailed Student’s t-test (g, j). Scale bars, 50 μm.
