Extended Data Fig. 2: M-FISH and presynaptic tracing of Opn5 POA neurons.
From: Violet-light suppression of thermogenesis by opsin 5 hypothalamic neurons
a, b, Representative images of Opn5cre/+;Ai14 POA neurons probed for Ptgds (green), tdTomato (red), Trpm2 (blue) and labelled with DAPI for nuclei (greyscale) (a) with corresponding quantification of overlap (n = 3 mice; 92 cells) (b). c, Representative images of Opn5cre/+;Ai14 cells (red) also positive for Trpm2 (blue) but with Ptgds labelling (green) that is below the background labelling threshold. Scale bars, 5 μm (c), 25 μm (a). d, Schematic of the mouse genetics used for rabies viral tracing. e, Experimental timeline for POA-tracing, and primary infected neurons (yellow). f–o, Traced neurons (red) located in the paraventricular nucleus (PVN) (f–h), supraoptic nucleus (SON) (f, g, i), dorsomedial hypothalamus (DMH) (j, k), lateral parabrachial (LBP) (l, m), and raphe pallidus (RPa) (n, o). Green regions in g and i are optic tracts with axons from Opn5 retinal ganglion cells. p, Schematic representation of nuclei presynaptic to Opn5 POA neurons. Data in b are mean ± s.e.m. Scale bars, 5 μm (c), 25 μm (a), 75 μm (e), 100 μm (h, i, k, o) or 200 μm (g, m). 2Cb, lobule 2 of cerebellar vermis.
